FIG. 6.

Colocalization of mtDNA and the Hmi1 protein or the Hmi1 mutants in situ. Yeast cells expressing the tagged Hmi1 protein or the C-terminal deletion mutants were fixed, and indirect immunostaining was used to analyze the intracellular localization of the Hmi1 protein or its mutants (right panels). mtDNA was stained in the same samples by using DAPI (left panels). Panels 1 and 2, W303-1A; panels 3 and 4, W303-1A transformed with YCAH-HMI1; panels 5 and 6, W303-1A transformed with YCAH-ΔC15Ala; panels 7 and 8, W303-1A transformed with YCAH-ΔC33Gly.