| Gene (Drosophila melanogaster) |
sha |
FlyBase |
FlyBase ID:FBgn0003382 |
|
| Gene (Drosophila melanogaster) |
bond |
FlyBase |
FlyBase ID: FBgn0260942 |
|
| Genetic reagent (Drosophila melanogaster) |
NM FRT40A |
BDSC BL-1835 |
RRID:BDSC_1835
|
Control for MARCM clones |
| Genetic reagent (Drosophila melanogaster) |
FRT82B NM |
PMID:23079600
|
|
Control for MARCM clones |
| Genetic reagent (Drosophila melanogaster) |
FRT42D tub-GAL80 |
PMID:28414313
|
|
For 2R MARCM clones |
| Genetic reagent (Drosophila melanogaster) |
FRT82B tub-GAL80 |
BDSC BL-5135 |
RRID:BDSC_5135
|
For 3R MARCM clones |
| Genetic reagent (Drosophila melanogaster) |
FRT42D act-GAL80 tub-GAL80
|
PMID:33135631
|
|
For 2R MARCM clones at 29C |
| Genetic reagent (Drosophila melanogaster) |
yw hs-Flp, UAS-nGFP, tub-GAL4
|
PMID:33135631
|
|
For MARCM clones |
| Genetic reagent (Drosophila melanogaster) |
ubi-GFP FRT40A
|
BDSC BL-5189 |
|
|
| Genetic reagent (Drosophila melanogaster) |
ubi-GFP FRT40A FRT42B His2Av-RFP/tub-lacZ FRT40A FRT42B
|
PMID:28414313
|
|
For multicolor clones |
| Genetic reagent (Drosophila melanogaster) |
C587-GAL4 |
BDSC BL-67747 |
RRID:BDSC_67747
|
GAL4 expressed in ECs and FSCs |
| Genetic reagent (Drosophila melanogaster) |
UAS-FUCCIOn second chromosome |
BDSC BL-55121 |
|
Nuclear GFP and RFP FUCCI |
| Genetic reagent (Drosophila melanogaster) |
UAS-FUCCIOn third chromosome |
BDSC BL-55122 |
|
Nuclear GFP and RFP FUCCI |
| Genetic reagent (Drosophila melanogaster) |
bond-GAL4/G-TRACE
|
PMID:31919193
|
|
Dr. Ruth Lehmann (MIT) |
| Antibody |
Anti-GFP (rabbit polyclonal) |
Molecular Probes |
A6455 |
(1:1000) |
| Antibody |
Anti-Fas3 (mouse monoclonal) |
Developmental Studies Hybridoma Bank(DSHB) |
7G10 |
(1:250) |
| Antibody |
Anti-Lamin-C (mouse monoclonal) |
DSHB |
LC28.26 |
(1:50) |
| Antibody |
Anti-Hts (mouse monoclonal) |
DSHB |
1B1 |
(1:20) |
| Antibody |
Anti-Traffic Jam (guinea pig polyclonal) |
PMID:23720044
|
RRID:AB_2567862
|
Dr. Dorothea Godt (University of Toronto)(1:5000) |
| Antibody |
Anti-Zfh-1 (rabbit polyclonal) |
Ruth Lehmann |
|
Dr. Ruth Lehmann (MIT)(1:5000) |
| Antibody |
Anti-Castor (rabbit polyclonal) |
PMID:9436984
|
|
Dr. Ward Odenwald (National Institutes of Health) (1:1000) |
| Antibody |
Anti-Vasa (rabbit polyclonal) |
Ruth Lehmann |
|
Dr. Ruth Lehmann (MIT)(1:1000) |
| Antibody |
Alexa Fluor 488 goat anti-rabbit(polyclonal) |
Thermo FisherScientific |
Cat# A11034; RRID:AB_2576271
|
(1:1000) |
| Antibody |
Alexa Fluor 647 goat anti-rabbit(polyclonal) |
Thermo Fisher Scientific |
Cat# A21245; RRID:AB_2535813
|
(1:1000) |
| Antibody |
Alexa Fluor594 goat-anti mouse (polyclonal) |
Thermo Fisher Scientific |
Cat# A11032; RRID:AB_2534091
|
(1:1000) |
| Antibody |
Alexa Fluor 546 goat-anti mouse (polyclonal) |
Thermo Fisher Scientific |
Cat# A11030; RRID:AB_2534089
|
(1:1000) |
| Antibody |
Alexa Fluor 647 goat-anti mouse (polyclonal) |
Thermo Fisher Scientific |
Cat# 21236; RRID:AB_2535805
|
(1:1000) |
| Antibody |
Alexa Fluor 647 goat anti-rat (polyclonal) |
Thermo Fisher Scientific |
Cat# 21247; RRID:AB_141778
|
(1:1000) |
| Antibody |
Alexa Fluor 488 goat anti-guinea pig (polyclonal) |
Thermo Fisher Scientific |
Cat# A11073; RRID:AB_2534117
|
(1:1000) |
| Antibody |
Alexa Fluor 594 goat anti-guinea pig (polyclonal) |
Thermo Fisher Scientific |
Cat# A11076; RRID:AB_2534120
|
(1:1000) |
| Antibody |
Alexa Fluor 647 goat anti-guinea pig (polyclonal) |
Thermo Fisher Scientific |
Cat# A21450; RRID:AB_2735091
|
(1:1000) |
| Chemical compound, drug |
DAPI Fluoromount-G |
Southern Biotech |
0100-20 |
Mount samples and stain for DAPI |
| Commercial assay or kit |
Click-iT EdU Cell Proliferation Kit |
Thermo Fisher Scientific |
C10086 |
|
| Software, algorithm |
ZEN Blue, ZEN Black, and ZEN Lite |
Zeiss |
|
For viewing z-stack images and quantifying fluorescence |
