| Biological sample (Homo sapiens) |
Human umbilical vein endothelial cells (HUVEC) |
Hematology Center, University of Campinas, Campinas, SP, Brazil |
|
Primary cells isolated from the umbilical vein |
| Antibody |
ICAM-1 (mouse monoclonal) |
Abcam |
Cat. #ab2213;RRID:AB_302892
|
Antibody clone MEM-111IF (1:100) |
| Antibody |
VCAM-1 (mouse monoclonal) |
Abcam |
Cat. #ab212937;RRID:AB_2892824
|
Antibody clone 1.4C3IF (1:500)FC (1:100) |
| Antibody |
IgG1 isotype control (mouse monoclonal) |
Dako |
Cat. #X0931;RRID:AB_2892825
|
IF (1:10) |
| Antibody |
ICAM-1 (mouse monoclonal) |
BioLegend |
Cat. #322714;RRID:AB_535986
|
Antibody clone HCD54FC (1:100) |
| Antibody |
pLDH Vivax-specific (mouse monoclonal) |
Vista Diagnostics International LLC, WA |
Cat. #3h8;RRID:AB_2892826
|
Antibody clone 3h8ELISA (1 μg/mL) |
| Antibody |
pLDH detection antibody (goat monoclonal) |
Vista Diagnostics International LLC, WA |
Cat. #6c9;RRID:AB_2892827
|
Antibody clone 6c9ELISA (1:4000) |
| Sequence-based reagent |
qRT-PCR Oligonucleotides |
This study |
See Supplementary file 2
|
|
| Commercial assay or kit |
Customized multiplex suspension detection system |
R&D Systems |
|
|
| Commercial assay or kit |
Accutase Cell Detachment Solution |
BioLegend |
Cat. #423201 |
|
| Chemical compound, drug |
Fixable Viability Dye eFluor 506 |
ThermoFisher |
Cat. #65-0866-14 |
|
| Software, algorithm |
FlowJo software (v10) |
Ashland, OR |
|
https://www.flowjo.com
|
| Software, algorithm |
RStudio software (v1.4.1106) |
RStudio, Boston, MA |
|
https://www.rstudio.com
|
| Software, algorithm |
Cytoscape software (v3.8.1) |
NIGMS, Bethesda, MD |
|
https://cytoscape.org
|
| Software, algorithm |
GraphPad Prism 9 (v9.1.1 (223)) |
GraphPad Software, San Diego, CA |
|
graphpad.com
|
| Software, algorithm |
ImageJ software |
NIH, Bethesda, MD |
|
imagej.nih.gov
|
