Fig. 4.

LRRK2 deficiency in nonhematopoietic cells promoted profibrotic immune responses and the profibrotic polarization of mo-Macs upon bleomycin exposure. The four groups of BM chimeric mice were challenged with 1.5 mg/kg bleomycin for 7 d. (A) TGF-β1 expression in BALF were quantified by ELISA analysis (n = 6 to 10 mice per group). (B and C) Numbers of (B) mo-Macs and (C) neutrophils in lung tissues were quantified by flow cytometry (n = 16 to 19 mice per group). (D–F) Quantification of relative mRNA levels of profibrotic factors including (D) Chil3, (E) Arg1, and (F) Spp1 by qRT-PCR in isolated lung mo-Macs (n = 5 to 6 mice per group). Data are shown as mean ± SEM and are (A–C) integrated from or (D–F) representative of at least two independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001, by two-tailed Student’s t test.