Fig. 6.

LRRK2 deficiency led to increased cellular senescence in AT2 cells in the context of pulmonary fibrosis. WT and Lrrk2^−/− mice were treated with saline or 1.2 mg/kg bleomycin for 7 d (n = 3 to 4 mice per group). (A) Representative Western blot of p53 and p21 expression in isolated AT2 cells. (B–F) Quantification of relative mRNA levels of representative SASP factors, including (B) Ccl2, (C) Il6, (D) Serpine1, (E) Mmp2, and (F) Mmp10, in isolated AT2 cells by qRT-PCR. Three independent experiments were performed, and β-Actin was used as the loading control. Data are shown as mean ± SEM; *P < 0.05, **P < 0.01, ****P < 0.0001, by two-way ANOVA followed by Bonferroni’s multiple comparison test (B–F).