Fig. 7.

The enhanced recruitment of mo-Macs and aberrant profibrotic responses were CCL2/CCR2 dependent in LRRK2-deficient mice. (A) ELISA analysis of CCL2 concentration in BALF collected from indicated BM chimeric mice treated with 1.5 mg/kg bleomycin for 7 d (n = 6 to 10 mice per group). (B–H) WT, Lrrk2^−/−, Ccr2^−/−, and Ccr2^−/−Lrrk2^−/− mice were treated with 1.5 mg/kg bleomycin for 7 d, and BALF and lung tissues were harvested at day 7 for the following analyses. (B) Numbers of mo-Macs in lung tissues were quantified by flow cytometry (n = 8 to 11 mice per group). (C) TGF-β1 expression in BALF was quantified by ELISA analysis (n = 9 to 17 mice per group). (D) Representative images of H&E and Masson’s trichrome staining of lung tissue sections. (Scale bars: 100 µm.) (E) Quantification of collagen content in lung homogenates using the Sircol Assay (n = 14 to 17 mice per group). (F) Numbers of neutrophils in lung tissues were quantified by flow cytometry (n = 8 to 11 mice per group). (G and H) ELISA analysis of neutrophil chemoattractant (G) CXCL1 and (H) CXCL2 levels in BALF (n = 10 to 11 mice per group). Data are shown as mean ± SEM and are integrated from two to three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, two-tailed Student’s t test.