Fig. 1.

Evaluating immunosuppressive capacity of IFN-γ–licensed MSCs on hydrogel biomaterials. Experimental setup with (Upper Left) list of RoosterBio human bone marrow–derived MSC cell lines tested from various donors, (Lower Left) list of tested biomaterial hydrogels, and (Right) timeline of experimental procedures. After overnight culture on the biomaterials, the MSC cell lines were licensed with IFN-γ for 24 h and then cocultured with CFSE-stained PBMCs activated with anti-CD3/CD28 dynabeads. After 72 h of coculture, the proliferation and activation of the CD4⁺ and CD8⁺ T cells in the PBMCs were measured with flow cytometry analysis.