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. 2021 Aug 25;118(35):e2026583118. doi: 10.1073/pnas.2026583118

Fig. 3.

Fig. 3.

ImFCS but not DRM detects subtle stabilization of Lo-like regions in Ag-stimulated RBL cells. (A) Degree of detergent resistance for PM-EGFP, EGFP-GG, and S15-EGFP and R values for −Ag (red) and +Ag (black) conditions. Box plots of fluorescence values of individual cells for −/+TX100 and −/+Ag conditions for these probes are provided in SI Appendix, Fig. S3. (B–D) A total of 30 bootstrapped CDFs of D values from ImFCS measurements are overlaid for specified probes and conditions (−/+Ag). Box plots of all D values and distribution of stimulated %change of Dav as described for Fig. 2E. SI Appendix, Table S1 shows number of autocorrelation function and cells measured for ImFCS analyses.