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Mn stabilizes and induces nuclear accumulation of HIF1-α and HIF2-α protein in HepG2 cells. (A) Immunoblot analyses after treatment with 0 or 250 µM Mn for indicated times. (B and C) Quantification of HIF1-α or HIF2-α levels normalized to tubulin from A. n = 3. Mean ± SE, *P < 0.05 by one-way ANOVA and Dunnett’s post hoc test for comparisons between 0 h and other groups. (D) Immunofluorescence after treatment with or without 250 µM Mn for 4 h.
