Fig. 6.

Mn inhibits the prolyl hydroxylation of HIF1-α. (A) qRT-PCR analyses in HepG2 cells treated with 250 µM Mn for indicated times. Expression in cells that did not receive Mn was normalized to 1. n = 3. Mean ± SE, *P < 0.05 by one-way ANOVA and Dunnett’s post hoc test for comparison between no Mn and other groups. (B) Immunoblot analyses of whole-cell lysates obtained from HepG2 cells treated with or without 10 µM MG132 for 1 h followed by exposure to 0 or 250 µM Mn for 4 h. HIF1-α–OH, prolyl hydroxylated HIF1-α.