Fig. 9.

Mn treatment up-regulates SLC30A10 in a HIF-dependent manner in primary human hepatocytes. (A and B) qRT-PCR assays in cells treated with or without 10 µM LW6 for 1 h followed by 0 or 250 μM Mn for 4 h (A) or with or without 20 μM roxadustat or 80 μM vadadustat for 16 h (B). For A, separately for cells that did or did not receive LW6, expression in the absence of Mn treatment was normalized to 1. For B, expression in the absence of drug treatment was normalized to 1. n = 3. Mean ± SE, *P < 0.05 by Student’s t test (A) or one-way ANOVA and Dunnett’s post hoc test for the comparison between no drug and other conditions (B).