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. 2021 Aug 25;118(35):e2023909118. doi: 10.1073/pnas.2023909118

Fig. 2.

Fig. 2.

Mitochondria show a swollen morphology in SLC30A9–deficient HeLa cells. (A) Representative confocal images of a HeLa cell coexpressing mitochondria-mKate and SLC30A9-GFP. The arrow indicates mitochondria. (Scale bar, 10 µm.) (B) Representative confocal images showing mitochondrial pattern and morphology, visualized by MitoTracker blue in HeLa WT, SLC30A9−/−, SLC30A9−/− SLC30A9-GFP, and SLC30A9−/− SLC30A9(A350Del)-GFP cells. The arrows show typical normal mitochondria, and the arrowheads indicate swollen mitochondria. (Scale bar, 10 µm.) (C) Quantification of the swollen mitochondrial phenotype of HeLa WT, SLC30A9−/−, SLC30A9−/− SLC30A9-GFP, and SLC30A9−/− SLC30A9(A350Del)-GFP cells. Data are shown as mean ± SEM. One-way ANOVA with Tukey correction. ***P < 0.001. One hundred and fifty cells or more were scored for each genotype. (D) TEM images of mitochondria in WT and SLC30A9−/− HeLa cells. (Scale bar, 500 nm.) The arrow points to a normal mitochondrion, and the arrowhead indicates a swollen mitochondrion.