TABLE 1.
Summary of RT-PCR products
| PCR primer paira | Product(s)c | Band size (bp)b |
|---|---|---|
| 4F-5R | Ex 4, Ex 5 | 202 |
| 3aF-3aR | In 3a | 449 |
| In 3a, spliced | 150 | |
| 3cF-3cR | In 3a, Ex E, In 3b/c-2 | 961 |
| In 3a, Ex E | 773 | |
| Ex E | 228 | |
| In 3c, spliced | 154 | |
| 3aF-3cR | In 3a, Ex D, Ex E | 1014 |
| In 3a, Ex D | 936 | |
| Ex D, Ex E | 711 | |
| Ex D | 637 | |
| Ex E | 243 | |
| In 3b spliced | 169 | |
| 6F-6R | In 6a, Ex I | 316 |
| In 6, spliced | 278 | |
| 6F-RV | 5′ In 6 | 434 |
| FV-6R | 3′ In 6a, Ex I, In 6b | 356 |
| 2F-2R | Not shown | 177 |
| rp49F-rp49R | Not shown | 318 |
a
For primer sequences, see reference and Materials and Methods.
b
Underlining indicates band expected from 116-kDa ORF transcript.
c
Figure 2B and C shows splicing profiles. In, intron; Ex, exon.