Figure 1.
Overexpression of NQO1 in NQO1-stable transfected cells. BEAS-2B cells stably transfected with pcDNA3.1 (Ctr), pCD-NQO1 (CMV-NQO1), pWT-NQONQO1 (NQO1-NQO1), and pmut-NQONQO1 (SNP) were incubated under room air (RA) or 80% O2 (O2) conditions for 48 h and subjected to qPCR using total RNA extracted from these cells. Gene expression of NQO1 (a), CYP1A1 (b), CYP1B1 (c), and AHR (d) were determined. ∗Statistically significant difference between room air and hyperoxia. †Statistically significant difference with Ctr. ‡Statistically significant difference between the NQO1-NQO1 and SNP-NQO1 promoter (n = 3; P < 0.05).