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. 2021 Oct 15;2021:5544600. doi: 10.1155/2021/5544600

Figure 1.

Figure 1

Overexpression of NQO1 in NQO1-stable transfected cells. BEAS-2B cells stably transfected with pcDNA3.1 (Ctr), pCD-NQO1 (CMV-NQO1), pWT-NQONQO1 (NQO1-NQO1), and pmut-NQONQO1 (SNP) were incubated under room air (RA) or 80% O2 (O2) conditions for 48 h and subjected to qPCR using total RNA extracted from these cells. Gene expression of NQO1 (a), CYP1A1 (b), CYP1B1 (c), and AHR (d) were determined. Statistically significant difference between room air and hyperoxia. Statistically significant difference with Ctr. Statistically significant difference between the NQO1-NQO1 and SNP-NQO1 promoter (n = 3; P < 0.05).