KEY RESOURCE TABLE

REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
53BP1	CST	4937S
53BP1	BD Pharmingen	612522
53BP1	NB	NB100-304
p53	SCBT	DO-1
CDKN1A/p21	CST	2947S
MDM2	SCBT	sc-965
β-Actin	SCBT	sc-47778
TIRR	Sigma	HPA044186
FLAG	sigma	M2 clone-F3165
GFP	CST	2555
p53	Invitrogen	MA5-12571
p21	SCBT	sc-756
p53 (mouse)	Thermofisher Scientific	MA5-12571
p21 (rabbit)	SCBT	sc-756
p53K382ac	Abcam	ab75754
SET8	CST	2996S
USP28	Abcam	EPR4249
y-H2AX	Millipore	05-636
Chemicals, Peptides, and Recombinant Proteins
Nutlin-3	Cayman Chemical	10004372
Dulbecco’s modified Eagle medium	Gibco	11995-065
Penicillin-Streptomycin	Gibco	15140
RNeasy mini extraction kit	Qiagen	74104
Superscript iii first strand synthesis system	Invitrogen	18080-051
Power Sybr green master mix	Applied Biosystems	4367659
Flag-M2 agarose beads	Sigma	A2220
GFP-trap	Chromotrek	GTA-20
DAPI	Sigma	D9542
Cycloheximide	Sigma	C7698
MG132	Selleckchem	S2619
Triton	Fisher	AC327371000
Tris-HCL	Invitrogen	15567-027
Glycine	Sigma	G8898
Resazurin	Sigma	R7017
Crystal violet	Millipore	C0775
Dynabeads CD25	Life Technologies	11157D
Pierce™ ChIP-grade Protein A/G Magnetic Beads	Thermofisher Scientific	26162
Lipofectamine™ RNAiMAX Transfection Reagent	Invitrogen	13778150
TrueCut™ Cas9 Protein v2	Thermofisher Scientific	A36497
Lipofectamine 2000	Invitrogen	11668019
MNase enzyme	Thermofisher Scientific	10107921001
Centrinone	Cayman chemicals	CFI-400945
PI/RNAse staining buffer	BD biosciences	550825
P53K370me2	(Cui et al., 2009)	N/A
P53k382me2	(Cui et al., 2009)	N/A
53BP1 TTD	(Botuyan et al., 2018)	N/A
Critical Commercial Assays
Duolink PLA red in situ mouse/rabbit starter kit	Sigma	DUO92101
Duolink PLA multi probe reagent kit	Sigma	DUO96000
TaqMan™ Array Human p53 Signaling	Thermofisher Scientific	4414168
Q5 site directed mutagenesis kit	NEB	E0554S
Beta galactosidase staining kit	CST	9860S
Deposited Data
RNA sequencing data in CT and TIRR KO	This paper	PRJNA661632
Experimental Models: Cell Lines
RPE1	ATCC	ATCC® CRL-4000™
RPE1 CT	This paper	N/A
RPE1 TIRR −/− #3	This paper	N/A
RPE1 TIRR −/− #5	This paper	N/A
RPE1 53BP1 −/−	(Drane et al., 2017)	N/A
RPE1 p53 −/−	This paper	N/A
U2OS	ATCC	ATCC® HTB-96™
U2OS TIRR −/− #5	This paper	N/A
U2OS TIRR −/− #17	This paper	N/A
Oligonucleotides
HPRT1, Actin, GAPDH MM	IDT	Housekeeping mastermixes
TP53 sgRNA #1: 1-F: ACCGGCAGTCACAGCACATGACGG	IDT	N/A
TP53 sgRNA #1: 1-R: AAACCCGTCATGTGCTGTGACTGCC	IDT	N/A
TP53 sgRNA #2: 2F:CACCGGCTTGTAGATGGCCATGGCG	IDT	N/A
TP53 sgRNA #2: 2R: AAACCGCCATGGCCATCTACAAGCC	IDT	N/A
TIRR sgRNA: CAGTGCCAAGATGTCGACGG(CGG)	IDT	N/A
53BP1 sgRNA: AGAACGAGGAGACGGUAAUAGUGGG	IDT	N/A
Primers for qRT-PCR, see Table S1	IDT	N/A
Recombinant DNA
pOZ-FH-N 53BP1 wt (full-length)	(Botuyan et al., 2018)	N/A
pOZ-FH-N 53BP1 wt (del BRCT)	(Drane et al., 2017)	N/A
pOZ-FH-N TIRR (WT)	(Botuyan et al., 2018, Drane et al., 2017)	N/A
pOZ-FH-N TIRR (K10E)	(Botuyan et al., 2018, Drane et al., 2017)	N/A
pOZ-FH-N TIRR (R107S)	(Botuyan et al., 2018)	N/A
GFP-p53	Addgene	Addgene plasmid # 12092
GFP-p53 (del CTD)	This paper	N/A
GFP-p53 (K382R)	This paper	N/A
Software and Algorithms
FlowJo	FlowJo	https://www.flowjo.com/
DEseq2	(Love et al., 2014)	https://github.com/mikelove/DESeq2
EdgeR	(Robinson et al., 2010)	https://bioconductor.org/packages/release/bioc/html/edgeR.html
IPA	Qiagen	https://digitalinsights.qiagen.com/products/ingenuitypathway-analysis;%20release%202019-06-15/
Modfit	Modfit	http://www.vsh.com/products/mflt/index.asp
Cell Profiler	Cell Profiler	https://cellprofiler.org/
Biorender	Biorender	https://biorender.com/
Rstudio v1.2.1335	Rstudio, Inc.	https://rstudio.com/
Graphpad Prism 7	Graphpad	https://www.graphpad.com/scientific-software/prism/