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Establishment of the validity of the Cas12a/crRNA reaction. A Sequences of crRNA2 for the target sites. B Visual detection under LED blue light. C Quantification of the maximum fluorescence signal of the Cas12a/crRNA reaction collected using the qPCR instrument (n = 3, error bars showed mean ± SEM). Statistical analyses were performed using the Student’s t test in GraphPad Prism
