Table 1.
The crRNA, primers, and probes used in this study
| Primers | Sequences (5′–3′) | Size/bp |
|---|---|---|
| ORF3-F | CTTGGACTTTTTCAATACACGATTG | 619 |
| ORF3-R | TCATTCACTAATTGTAGCATACTCGT | |
| RT-qPCR-F | ATTGCCCACTTTTATATTATTGTGG | 286 |
| RT-qPCR-R | TGCCGCCCACGTATAGCTAGATACA | |
| RT-ERA-F | TTCCAATTAGACAAGCTTCAAATGTGACGGG | 238 |
| RT-ERA-R | CGCCAGGAGTAAAAGCAGACTAAACAAAGCC | |
| crRNA-1-F | GAAATTAATACGACTCACTATAGGGTAATTTCTACTAAGTGTAGATTATTATTGTGGTGCATTTTT | 66 |
| crRNA-1-R | AAAAATGCACCACAATAATAATCTACACTTAGTAGAAATTACCCTATAGTGAGTCGTATTAATTTC | |
| crRNA-2-F | GAAATTAATACGACTCACTATAGGGTAATTTCTACTAAGTGTAGATGATGCAACTATTATTTGTTG | 66 |
| crRNA-2-R | CAACAAATAATAGTTGCATCATCTACACTTAGTAGAAATTACCCTATAGTGAGTCGTATTA ATTTC | |
| Probe | FAM-N6-BHQ1 |