Table 1.
RT-qPCR primers and target genes
| Target gene | Gene symbol | GenBank accession number | Primer/probe sequence | Exon location Fwd/Rv |
E (%) | Tm (°C) Fwd/Rv | Product size (bp) | Anneal temp (°C) |
|---|---|---|---|---|---|---|---|---|
| Arginine vasopressin | Avp | NM_009732.2 |
F: CTCAACACTACGCTCTCCGC R: CAGCAGATGCTTGGTCCGA |
1/1–2 | 98 | 60.8/57.9 | 173 | 55 |
| Arginine vasopressin receptor 1A | Avp1ar | NM_16847.2 |
F: GCTGGACACCTTTCTTCATCGTC R: CTGTTCAAGGAAGCCAGTAACG |
1/2 | 89.1 | 61.7/59.5 | 115 | 55 |
| Adenylate cyclase activating polypeptide 1 | Adcyap1 | NM_009625.3 |
F: AGGTGCTGGTGTTGGAATGAATGC R: AATGCATGAGGGCAAGGGTAGGAA |
5 | 95 | 60.2/60.7 | 176 | 55 |
| Adenylate cyclase activating polypeptide 1 receptor 1 | Adcyap1r1 | NM_007407.4 |
F: TTCACTACTGCGTGGTGTCCAACT R: ATATCCCAGCATCCCGCATCATCA |
10/11–12 | 96.3 | 60.3/60.3 | 199 | 55 |
| Oxytocin | Oxt | NM_011025.4 |
F: CCGAAGCAGCGTCTTTT R: CTTGGCTTACTGGCTCTGAC |
1/2 | 96.9 | 55.7/55.5 | 131 | 60 |
| Oxytocin receptor | Oxtr | NM_001081147.2 |
F: CGCACAGTGAAGATGACCTT R: ATGGCAATGATGAAGGCAGA P: 6-FAM-CTTCGTGCA-ZEN-GATGTGGAGCGTTCT-IBFQ |
1/2 | 107.1 | NA | 131 | 60 |
| Beta actin | β-Actin | NM_007393.5 |
F: GATTACTGCTCTGGCTCCTAG R: GACTCATCGTACTCCTGCTTG P: HEX-CTGGCCTCA-ZEN-CTGTCCACCTTCC-IBFQ |
5/6 |
99.6 101.7 |
55.0/54.4 NA |
147 | 60 |
Abbreviations: F forward, R reverse, P probe, E primer efficiency, Tm melting temperature, bp base pair, ZEN/IBFQ ZEN–Iowa Black FQ, FAM Fluorescein, HEX Hexachloro-fluorescein