REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Rat monoclonal anti-mouse-CD45R/B220 PE-CF594(clone RA3-6B2)	BD Biosciences	Cat#562290; RRID: AB_11151901
Rat monoclonal anti-mouse-CD19 APC-Cy7(clone 1D3)	BD Biosciences	Cat#557655; RRID: AB_396770
Rat monoclonal anti-mouse-IgM PE-Cy7(clone II/41)	Invitrogen	Cat#25-5790-82; RRID: AB_469655
Fluorescein (FITC)-AffiniPure Goat Anti-Mouse IgM, μ Chain Specific	Jackson Immunoresearch	Cat#115-095-075; RRID: AB_2338598
Rat monoclonal anti-mouse-IgD BV711 (clone 11-26c.2a)	BioLegend	Cat#405731; RRID: AB_2563342
Rat monoclonal anti-mouse-GL-7 PE (clone GL7)	BD Biosciences	Cat#561530; RRID: AB_10715834
Rat monoclonal anti-mouse-CD38 Alexa Fluor 700 (clone 90)	Invitrogen	Cat#56-0381-82; RRID: AB_657740
Rat monoclonal anti-mouse-CD8a PerCP-Cy5.5 (clone 53-6.7)	Invitrogen	Cat#45-0081; RRID: AB_1107004
Rat monoclonal anti-mouse-GL-7 FITC (clone GL7)	BioLegend	Cat#144603; RRID: AB_2561696
Rat monoclonal anti-mouse/human-IRF4 EF450 (clone 3E4)	Invitrogen	Cat#48-9858-82; RRID: AB_2574135
Biotin Rat Anti-Mouse IgG1(clone A85-1)	BD Biosciences	Cat#553441; RRID: AB_394861
Biotin Mouse Anti-Mouse IgG2a [b] (clone 5.7)	BD Biosciences	Cat#553504; RRID: AB_394889
Goat polyclonal Secondary Antibody to Monkey IgG - H&L (HRP)	Abcam	Cat#ab112767
Goat Anti-Mouse IgM-HRP	SouthernBiotech	Cat#1021-05; RRID: AB_2794240
Goat Anti-Mouse IgG2c, Human ads-HRP	SouthernBiotech	Cat#1079-05; RRID: AB_2794466
Goat Anti-Mouse IgG1, Human ads-HRP	SouthernBiotech	Cat#1070-05; RRID: AB_2650509
Goat Anti-Mouse IgG2b, Human ads-HRP	SouthernBiotech	Cat#1090-05; RRID: AB_2794521
Goat Anti-Mouse IgG3, Human ads-HRP	SouthernBiotech	Cat#1100-05; RRID: AB_2794573
Goat anti-Mouse IgA Heavy Chain Antibody HRP Conjugated	Bethyl Laboratories	Cat# A90-103P
Goat anti-Mouse IgG-Fc Fragment Antibody HRP Conjugated	Bethyl Laboratories	Cat# A90-131P
Goat anti-Mouse IgG-Fc Fragment Antibody Affinity Purified	Bethyl Laboratories	Cat# A90-131A
IFN gamma Monoclonal Antibody (clone AN-18), Functional Grade	Invitrogen	Cat#16-7313-85; RRID: AB_469247
IFN gamma Monoclonal Antibody (clone R4-6A2), Biotin	Invitrogen	Cat#13-7312-85; RRID: AB_466939
IgD Monoclonal Antibody (clone 11-26c), Biotin	Invitrogen	Cat#13-5993-82; RRID: AB_466860
BV650 streptavidin	BioLegend	Cat#405231
PE streptavidin	BioLegend	Cat#405204
HRP-conjugated streptavidin	Jackson Immunoresearch	Cat#016-030-084; RRID: AB_2337238
Bacterial and virus strains
E. coli BL21(DE3)	TransGen Biotech	Cat# CD601
SARS-CoV-2 (nCoV-2019BetaCoV/Wuhan/WIV04/2019)	The National Virus Resource, China	N/A
SARS-CoV-2 strain 107	The Guangdong Provincial Center for Disease Control and Prevention, Guangdong, China	N/A
Chemicals, peptides, and recombinant proteins
CpG-ODN	Shanghai Generay Biotech	Sequence:TCCATGACGTTCCTGACGTT
Isopropyl β-D-1-thiogalactopyranoside (IPTG)	Yeasen biotech, China	Cat#367-93-1
Polyethylenimine (PEI)	Polyscience	Cat#23966-1
3,3′-Diaminobenzidine tetrahydrochloride hydrate	Sigma-Aldrich	Cat# D5637-1G
3,3′,5,5′-Tetramethylbenzidine	Sigma-Aldrich	Cat#860336-1G
Fast Red	Sigma-Aldrich	Cat# F8764-1G
D-biotin	BBI Life Sciences	Cat# A100340-0500
Imject Alum	Thermo	Cat# 77161
RBD peptides pool1	Smith, 2020 #31	N/A
RBD peptides pool2	Smith, 2020 #31	N/A
Recombinant AP205 protein	This paper	N/A
Recombinant AP205-SpyTag protein	This paper	N/A
Recombinant RBD protein	This paper	N/A
Recombinant RBD-SpyCatcher protein	This paper	N/A
Recombinant RBD-AviTag protein	This paper	N/A
Recombinant GST-BirA protein	This paper	N/A
Recombinant S protein	This paper	N/A
Critical commercial assays
Toxin Sensor Chromogenic LAL Endotoxin Assay Kit	GenScript	Cat# L00350C
Alexa Fluor 647 Protein Labeling Kit	Invitrogen	Cat# A20173
THUNDERBIRD Probe One-Step qRT-PCR kit	TOYOBO	Cat# QRZ-101
High Pure Viral RNA Kit	Roche	Cat#11858882001
TRIzol Plus RNA Purification Kit	Thermo Fisher	Cat# A33254
MiniBEST Viral RNA/DNA Extraction Kit	TaKaRa	Cat#9766
PrimeScript RT reagent Kit with gDNA Eraser	Takara	Cat# RR047A
Ni-NAT 5mL (Pre-Packed Gravity Column)	BBI Life Sciences	Cat# C600793-0010
GST Fusion Protein Purification Kit	GenScript	Cat# L00207
Bright-Glo Luciferase Assay Vector System	Promega	Cat# E2650
Experimental models: Cell lines
Expi293F	GIBCO	Cat# A14635
Vero-E6	ATCC	CRL-1586; RRID: CVCL_0574
Human: HEK293T	ATCC	Cat# CRL-11268
Human: Huh7	NICR	Cat#1101HUM-PUMC000679
Experimental models: Organisms/strains
C57BL/6	The Institute of Biophysics, Chinese Academy of Sciences	N/A
Rhesus macaques	Kunming Institute of Zoology (KIZ), Chinese Academy of Sciences (CAS)	N/A
Oligonucleotides
SARS-CoV-2- forward primer: 5′-GGGGAACTTCTCCTGCTAGAAT-3′	Song, 2020 #37	N/A
SARS-CoV-2- reverse primer: 5′-CAGACATTTTGCTCTCAAGCTG-3′	Song, 2020 #37	N/A
SARS-CoV-2-probe FAM-TTGCTGCTGCTTGACA GATT-TAMRA-3′	Song, 2020 #37	N/A
SARS-CoV-2-S- forward primer: 5′-CAATGGTTTAACAGGCACAGG-3′	This paper	N/A
SARS-CoV-2-S-reverse primer: 5′-CTCAAGTGTCTGTGGATCACG-3′	This paper	N/A
Recombinant DNA
pET21-AP205	This paper	N/A
pET21-AP205-SpyTag	This paper	N/A
pCEP4-RBD	This paper	N/A
pCEP4-RBD-SpyCatcher	This paper	N/A
pCEP4-RBD-AviTag	This paper	N/A
pGEX-BirA	This paper	N/A
pcDNA3.1-SARS-Cov-2 S	Ju, 2020 #22	N/A
Software and algorithms
FlowJo software Version 10.7.1	Tree Star	https://www.flowjo.com/
GraphPad Prism 8.0	GraphPad Software	https://www.graphpad.com/