Table 12.

LOD, LOQ and recovery of the GSH and GSSG test procedures used

Test system		LOD (nM)	LOQ (nM)	cnominal (µM)	Cdeterminedt (µM)	Recovery (%)
Glutathione recycling assay	tGSH	49 (n = 12)	98	640	659–680 (n = 3)	103–106 (n = 3)
				200	181–216 (n = 6)	90–108 (n = 6)
	GSSG	72 (n = 12)	144	20	18,9–24,1 (n = 8)	94–120 (n = 8)
NPSH	GSH	1041 (n = 27)	2082	480	470,0 ± 28,3 (n = 3)	91–102 (n = 3)

LOD, limit of detection; LOQ, limit of quantification; GSH, glutathione (reduced); GSSG, oxidized glutathione; tGSH, total glutathione (sum of GSH + GSSG); NPSH, non-protein sulfhydryl; C, concentration