Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Sep 8;35(Suppl 2):112–134. doi: 10.1007/s12028-021-01326-w

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 4 — Direct relationship between altered light transmittance (∆LT) and osmolality. a ∆LT can be imaged through a brain slice to indirectly measure cell swelling or shrinkage. Inverse relationship between light transmittance and aCSF osmolality is imaged in a rat neocortical slice. Gray matter LT increases in hypoosmotic aCSF (− 10 to − 80 mOsm) and completely reverses within 8 min of return to control aCSF (not shown). Conversely, LT decreases as osmolality is increased using mannitol (+ 10 to + 80 mOsm). The signal is primarily astrocytic, given that neuronal volume resists osmotic shifts within this pathophysiological range. Unpublished figure. b Plot of the mean peak change in LT versus shift in osmolality from a control level of 287–289 mOsm [2]. Slices (n = 9) display an inverse relationship between change in osmolality and ∆LT in all neocortical layers. From Andrew et al. [2]. aCSF, artificial cerebrospinal fluid, LT, light transmittance, ∆LT, change in light transmittance, ∆T, change in transmittance, mOsm, milliosmole.