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. 2021 Oct 22;12:6153. doi: 10.1038/s41467-021-26207-w

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — a Growth assay of isogenic wild-type, single snr190-[mut.C], single dbp7Δ and double dbp7Δ, snr190-[mut.C] mutants in the W303 background. Serial dilutions of these strains were grown on YPD plates at the indicated temperatures for 3–5 days. b Accumulation levels of rRNA precursors in the wild-type BY4741 strain or in two independent clones (#1 and #2) of the dbp7Δ strain and the dbp7Δ strain further manipulated using the CRISPR-Cas9 approach to deplete snR190 (snr190-[mut.C]) or to express snr190-[mut.A] and snr190-[mut.G34C]. Experiments were performed as explained in the legend of Fig. 2b. c Quantification of the 27SB/27SA₂ ratios for the indicated strains from the precursor levels measured in b using PhosphorImager data and the MultiGauge software. Data correspond to two biological replicates. Histograms represent the mean values. The individual data points are shown.