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. 2021 Oct 22;11:20888. doi: 10.1038/s41598-021-00006-1

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Species authentication by Bar-cas12a in admixture samples. (A) DNA amplification of DNA admixture in different proportions by RPA using trnL loci. DNA admixture between P. amarus (PA) and P. urinaria (PU) were done in different amount proportions as 100%, 50%, 25%, 10%, 5%, 2% and 0% of PA contaminated with PU which stock concentration of each species used was 10 ng/µl. (B,D) In vitro digestion of cas12a using gRNA A and gRNA B and observed under LED transilluminator. (C,E) the acquisition of fluorescence signal by realtime PCR over two hours. Initial images of agarose gel electrophoresis are shown in Figure S4.