Fig. 2. CHD8 depletion alters the apoptosis, cell cycle, and proliferation process in human ESCs.

A Apoptosis assay with PI and AnnexinV double staining detected by FACS. The results were calculated and compared with WT ESCs (n = 7). B Cell cycle analysis using PI staining in WT and CHD8-KO ESCs. The populations of different phases were calculated in FlowJo (v10.4.0) (n = 3). C The OD490 value for MTT assay in three time points during culture (n = 3). D Colony-forming analysis visualized by AP staining and the statistics of the colonies number was shown in bar graph (n = 3). The error bars represented the mean ± SD and the significance level was calculated by Student’s t-test (two-tailed, equal variance) (ns means not statistically significant, *P < 0.05, **P < 0.01, ***P < 0.001).