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. 2021 Oct 22;11:20886. doi: 10.1038/s41598-021-00389-1

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Representative cross-sectional images of the sciatic nerve upon toluidine blue staining in the compression and decompression phase (a) (× 400; scale bar = 50 μm; Sham, nerves of sham-operated mice; PBS, nerves of untreated mice during compression phase; CF, nerves of mice treated with clemastine during compression phase; d-PBS, nerves of untreated mice after surgical decompression; d-CF, nerves of mice treated with clemastine after surgical decompression). Comparison of the axoglial diameter, the axonal diameter, the G-ratio and the myelin thickness among sham, PBS, and CF group in compression phase (b). Comparison of these measurements among sham, PBS, and d-PBS group for evaluation of decompression effect (c). Comparison of these measurements among sham, d-PBS, and d-CF group in decompression phase (d). Error bars represent standard error of mean. One-way ANOVA with Turkey’s post-hoc test was conducted (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, and n = 300 axons from 3 animals per group).