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. 2021 Oct 22;11:20886. doi: 10.1038/s41598-021-00389-1

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Representative Western immunoblotting for P0 and MAG protein in compression phase (a) and decompression phase (b) (P0, myelin protein 0; MAG, myelin associated glycoprotein; Sham, nerves of sham-operated mice; PBS, nerves of untreated mice during compression phase; CF, nerves of mice treated with clemastine during compression phase; d-PBS, nerves of untreated mice after surgical decompression; d-CF, nerves of mice treated with clemastine after surgical decompression). Full-length Western blots are presented in Supplementary Figs. 1 and 2. Band intensity quantification of P0 and MAG of CF group as fold change of PBS group. Band intensity quantification of P0 and MAG of d-CF group as fold change of d-PBS group. ß-actin was used to normalize the intensity of the bands. Error bars represent standard error of mean. Mann–Whitney U test was conducted (*p < 0.05, and n = 3 or 4 animals per group).