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. 2021 Oct 22;11:20946. doi: 10.1038/s41598-021-00450-z

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Quantification of cell spreading dependent on actin-cytoskeleton. Cells were stained with phalloidin, and the area of cell spread was calculated. (a) top: Confocal images of splenocytes, bottom: corresponding spread area data from confocal images (n = 4, mice; 185 WT, 158 KO cells); (b) top: confocal images of BMDM, bottom: corresponding spread area data from confocal images (n = 7 mice; WT BMDM, 572, KO BMDM, 623); (c) top: confocal images of Raw 264.7 transfected macrophages, corresponding spread area data from confocal images (254, Control vector and 221, TBC1D10C cells; 2 independent experiments). Confocal images are presented as the sum of slice projections. Data were analyzed by the Mann–Whitney test (P < 0.05), and graphs show SEM (standard error of the mean).