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. 2021 Oct 22;11:20946. doi: 10.1038/s41598-021-00450-z

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Protein–protein interaction (PPI) analysis of TBC1D10C-interacting proteins and measurement of cell membrane stiffness. (a) The TBC1D10C interaction network was built from the STRING database with a confidence score > 0.4. The network contains 28 nodes and 90 edges. PPI analysis indicated multiple TBC1D10C-associated complexes. TBC1D10C is highlighted in bold capital letters; (b) membrane stiffness of TBC1D10C-transfected Raw264.7 macrophages was determined by atomic force microscopy (AFM). (20, control vector; 30, TBC1D10C; 16 TBC1D10C GAP mutant). Data were analyzed by the Mann–Whitney test (P < 0.05), and the graph shows SEM.