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. 2021 Oct 22;11:20891. doi: 10.1038/s41598-021-99805-9

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Ocular surface natural killer cells subsets and T cell subsets proportions in KC patients: The graphs indicate the percentage of CD45⁺CD56^Total (Total Natural Killer—NK) cells (a), CD45⁺CD56^Low—cytotoxic NK cells (b) and CD45⁺CD56^High—cytokine producing low cytotoxic NK cells (c) within the leukocyte population in ocular surface wash samples of eyes with different grades of KC. The graph in panel (d) represent the ratio of cytotoxic NK cells to cytokine producing NK cells—CD56^High/CD56^Low and the graph in panel (e) represent the ratio of neutrophils to NK cells within the leukocyte population in ocular surface wash samples of eyes with different grades of KC. The graphs indicate the percentage of CD45⁺CD3⁺—Pan-T cells (f), CD45⁺CD3⁺CD56⁺ (Natural Killer T) cells (g) and CD45⁺CD3⁺γδTCR⁺ (gamma delta) T cells (h) within the leukocyte population in ocular surface wash samples of eyes with different grades of KC. Ctrls—Controls; FFKC—Forme fruste keratoconus; All-KC—all the grades of keratoconus combined; SEM—standard error of the mean; Controls (23 eyes), All-KC (51 eyes), FFKC (6 eyes), grade 1 KC (14 eyes), grade 2 KC (8 eyes), grade 3 or 4 (23 eyes); Bar graphs represent Mean ± SEM; *P < 0.05, **P < 0.01,***P < 0.001, ****P < 0.0001, Mann–Whitney test.