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. 2021 Oct 22;11:20922. doi: 10.1038/s41598-021-00370-y

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Mitochondrial respiration activities and amino acid contents in leaf blades grown under control and Mn-toxic conditions. (a) Leaf respiration rate (n = 12–13) in the dark and (b) sucrose feeding respiration activities and mitochondrial respiratory electron transport activities depending on ATP synthase (uncoupled), COX, and AOX (n = 7). (c) Amino acid content in leaves sampled in the middle of the night (n = 6). (d–i) Enzyme activities involved in the TCA cycle (n = 13–14). Data are shown as box plots, black squares indicate the mean value, and bars indicate the range of the maximum or minimum data within a 1.5 × interquartile range (IQR). All data were obtained from independent biological replicates according to the indicated numbers. The green boxes indicate the results under control conditions, and the red boxes indicate those under Mn-toxic conditions. Asterisks represent significant differences between the control and Mn-toxic conditions (*p < 0.05, **p < 0.01, Kruskal–Wallis test).