Skip to main content
. 2021 Oct 22;4:1214. doi: 10.1038/s42003-021-02735-5

Fig. 7. CXCL1/2 administration reverses salutary effects of adipocyte CRTC2/3 depletion on glucose homeostasis and pro-inflammatory gene expression.

Fig. 7

a Effect of recombinant CXCL1/2 (rCXCL1/2) on CRTC dAKO mediated glucose tolerance following 12 weeks of HFD (1 g/kg of glucose). 1 µg of rCXCL1 and 2 were administered by IP injection 1 week prior to the IPGTT assay. Bottom, AUC of glucose tolerance test (*P < 0.05, one-way ANOVA, n = 5 per group). b Immunoblots of eWAT showing the effect of rCXCL1/2 on insulin and stress signaling as well as SIK2 protein amounts in eWAT from HFD-fed (12 weeks) WT and dAKO mice (n = 5 per group). Bottom, densitometric analysis of p-Akt, p-JNK and SIK2 expression (**P < 0.01, *P < 0.05, one-way ANOVA; n = 5 per group). c Effect of TNFα, α-CXCL1/2, and rCXCL1/2 on LPS-primed Raw264.7 migration. Adipocytes from WT and dAKO mice were treated overnight with 10 ng/ml TNFα, 100 ng/ml α-CXCL1/2, and 100 ng/ml rCXCL1/2. Following 16 h exposure to the adipocyte conditioned medium, Raw 264.7 macrophage migration was evaluated by staining inserts with crystal violet. Dried inserts were photographed and dyes were extracted with alcohol/acetic acid and quantified by OD measurement at 600 nm. Bottom, relative absorbance shown (**P < 0.01, t-test; n = 3 per group). Data in a–c represent the mean ± SEM. d Diagram showing the effect of HFD feeding on CRTC2/3 activation and induction of cytokine genes in cooperation with NF-κB.