Figure 2.

Assessment of repair and cell cycle state at seven or 14 days after a single cisplatin injection at a dose of 6 or 10 mg/kg. (a) Experimental scheme. A single dose of 6 mg/kg or 10 mg/kg of cisplatin was injected. The kidneys were assessed at seven or 14 days after the injection. (b) Histological analysis of the kidney. PAS staining, and immunostaining of Ki-67. (c, d) Quantification of the tubular injury score and the number of Ki-67 + tubules. (e) Quantitative PCR of whole kidneys for the cell cycle markers (Pcna, Fen1, CDK1, and Top2a). Data are the mean ± SE. *P < 0.05, †P < 0.05 vs other groups. Scale bar = 100 μm.