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. 2021 Oct 22;11:20880. doi: 10.1038/s41598-021-00306-6

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Interaction between ZSWIM8 and Cdon. (A) Schematic representation of full-length (FL) or C-terminally-deleted Cdon (ΔC; 1–1162, ΔMC; 1–1073, Δ983–1012, Δ1013–1042, and Δ1043–1073). The black box indicates the transmembrane (TM) region. (B) The juxtamembrane region of Cdon is recognized by ZSWIM8. HEK293T cells were transfected with empty plasmid or plasmid encoding non-tagged Cdon (FL, ΔC, or ΔMC) with or without 3 × FLAG-ZSWIM8 and cultured for 2 days. The cell lysates were immunoprecipitated with an anti-Cdon-N or FLAG antibody and immunoblotted with an anti-Cdon-N or FLAG antibody. Representative data of three independent experiments. (C) Deletion of the juxtamembrane region of Cdon did not affect the interaction with ZSWIM8. The experiment was performed as in (B). The membranes in (B) and (C) were cut prior to hybridization with antibodies. Full-length blots are presented in Supplementary Fig. 8.