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. 2021 Oct 22;11:20880. doi: 10.1038/s41598-021-00306-6

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Co-localization of ZSWIM8 and Cdon. (A) 3 × FLAG-ZSWIM8-expressing C2C12 cells were immunostained by anti-FLAG and Cdon-N antibodies. Nuclei were stained with DAPI. Scale bar, 10 μm. Representative colocalization in growing cells is indicated by white arrows. Representative data of two independent experiments. (B) Quantification of colocalization-positive cells in (A). Colocalization-positive cell number was counted and divided by total cell number in four different fields. Data represent the mean ± SD (n = 44).