. 2021 Oct 22;4:1215. doi: 10.1038/s42003-021-02748-0

Table 2.

Primer pairs designed in this study to target specific SARS-CoV-2 RNAs.

SARS-CoV-2 target	Primer name	Sequence (5′→3′)	Amplicon length	Genome coordinates (NC_045512v2)
ORF1ab RNA	ORF1ab For	AGATCTGAGGACAAGAGGGCA	158 bp	12314−12334
ORF1ab RNA	ORF1ab Rev	TTGGCTGCTGTTGTAAGAGGTA	158 bp	12471−12450
N sgmRNA	LS_For1	CGATCTCTTGTAGATCTGTTC	205 bp	44−64
N sgmRNA	N_Rev	AGCGGTGAACCAAGACGCA	205 bp	28438−28420
S sgmRNA	LS_For2	CCAACTTTCGATCTCTTGTAG	207 bp	36−56
S sgmRNA	S_Rev	AGAACAAGTCCTGAGTTGAATG	207 bp	21728−21707