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. 2021 Oct 22;4:1213. doi: 10.1038/s42003-021-02719-5

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — a Immunostaining with specific marker for ventral neural progenitor cells (NKX2.1, green) on Day 56. Organoids generated by vertical mixing exhibited NKX2.1-positive cells. Scale bars = 200 µm. b Immunostaining with specific markers for GABAergic neurons (GABA, red) and pan-neuron markers (MAP2, green) in brain organoid generated by orbital mixing and by vertical mixing on Day 90. Vertical mixing enriched GABA-positive cells. Scale bars = 50 µm.