Mounting Medium

Reagent	Final concentration	Amount
Tris, pH 8.5 (1 M)	100 mM	5 mL
DAPI Solution (1 mg/mL)	0.3 μg/mL	15 μL
Mowiol 4-88	10% (w/v)	5 g
1,4-Diazabicyclo [2.2.2] octane	1% (w/v)	0.5 g
Glycerol	25% (v/v)	12.5 mL
ddH2O	n/a	32.5 mL
Total	n/a	50 mL

Store at −20°C for 12 months. The medium remains stable for 1 month when stored at 4°C.