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. 2021 Jul 17;12(5):1599–1615. doi: 10.1016/j.jcmgh.2021.07.002

Figure 5.

Figure 5

LAMP2 reduction is a direct downstream event of ER stress in hepatocytes. (A) Hepa-1c1c7 cells were treated with tunicamycin (TM) at 5 μmol/L for 24 hours and immunoblotted for cellular LAMP2, ATF4, and CHOP. (B) ATF4 and LAMP2 were visualized by immunofluorescence microscopy under tunicamycin treatment. Scale bars, 20 μm. (C) Hepa-1c1c7 cells, with or without transfection of ATF4 knockdown CRISPR plasmid, were treated with 100 μmol/L PA for 24 hours and immunoblotted for cellular ATF4, CHOP, LAMP2, and LC3II. Data are shown as means ± SD. ∗P < .05.