Figure 8.

Pharmacologic activation of PPARα induces FA metabolism and reverses alcohol-induced liver injury. DGAT1^Δhep mice were PF or AF with or without Wy14,643 supplementation at 10 mg/kg/day starting from the last week in 8-week feeding experiment. (A) Immunoblot and quantification analysis of hepatic protein levels of ACADL, ACADM, ACSL1, and ACOX1 (n = 4/group). (B) Analysis of hepatic TG and FFA contents (n = 4/group). (C) Analysis of serum ALT and AST levels (n = 4/group). (D) H&E staining of liver tissue sections (n = 4/group). Arrowheads: hepatocyte degeneration. Arrows: hepatic lipid droplets. Scale bars, 20 μm. (E) Immunoblot and quantification analysis of protein levels of ATF4, CHOP, LAMP2, and LC3II (n = 4/group). (F and G) Immunohistochemistry staining of CHOP and LAMP2 on liver tissue sections (n = 4/group). Scale bars, 20 μm. Data are shown as means ± SD. ∗P < .05.