REAGENT or RESOURCE	SOURCE	IDENTIFIER
Bacterial and virus strains
NEB® 10-beta Competent E. coli (High Efficiency)	New England Biolabs Inc	Cat#C3019H
BL21(DE3) Competent E. coli	New England Biolabs Inc	Cat#C2527H
Chemicals, peptides, and recombinant proteins
Isopropyl β-D-1-thiogalactopyranoside (IPTG)	UBP Bio	Cat#P1010-100
β-Mercaptoethanol	Sigma-Aldrich	Cat#M3148-25ML
Phenylmethylsulfonyl fluoride (PMSF)	RPI	Cat#P20270-25.0
Ampicillin sodium salt	Fisher Scientific	Cat#BP1760-25
Kanamycin sulfate, ultrapure	Thermo Scientific	Cat# J1792414
Ubiquitin-Rhodamine 110 (Ub-Rho)	R&D Systems	Cat#U-555-050
Deposited data
Primary and dose response confirmation screening data	PubChem	https://pubchem.ncbi.nlm.nih.gov/bioassay/1645869
Recombinant DNA
pOPINK-OTUD3 (OTU+UBA, aa 52-275)	(Mevissen et al., 2013)	Addgene plasmid# 61411
pET28aLIC-USP7 (His-tagged, aa 207-532)	(Varca et al., 2021)	N/A
pET28aLIC-USP8 (His-tagged, aa 742-1110)	(Varca et al., 2021)	N/A
pET28PP-USP10 (His-tagged, aa 376-798)	(Varca et al., 2021)	N/A
pET28b-USP17 (His-tagged, aa 1-530)	(Varca et al., 2021)	N/A
pET28a USP25 (His-tagged, aa 207-532)	(Varca et al., 2021)	N/A
pET28aLIC-USP28 (His-tagged, aa 207-532)	(Varca et al., 2021)	N/A
pET28PP-USP30 (His-tagged, aa 207-532)	(Varca et al., 2021)	N/A
pGEX6P1-UCHL1 (GST-tagged, aa 1-223)	(Varca et al., 2021)	N/A
Software and algorithms
Prism 8	GraphPad	https://www.graphpad.com/scientific-software/prism/; RRID: SCR_002798
JMP	SAS Institute	RRID:SCR_014242
Other
Ni-NTA Agarose	QIAGEN	Cat#30210
Pierce™ Glutathione Superflow Agarose	Thermo Scientific	Cat#25237
QIAprep Spin Miniprep Kit	QIAGEN	Cat#27104
Amicon® Ultra-15 Centrifugal Filter Unit	Millipore Sigma	Cat#UFC903024
Fisher Scientific Model 505 Sonic Dismembrator	Fisher Scientific	Cat#FB505
NanoDrop 2000	Thermo Scientific	Cat#ND-2000
384-Well plates	Greiner	Cat#784201
1536-Well plates	Greiner	Cat#782076
Echo	Labcyte	Cat#ECHO555
Multidrop™ Combi Reagent Dispenser	Thermo Fisher Scientific	Cat#5840300
PHERAstar	BMG Labtech	Cat#PHERAstarFSX
Tecan Freedom EVO 200	Tecan	Cat#Freedom EVO 200
TEMPEST liquid handler	Formulatrix	Cat#TEMPEST