Figure 3.

Effects of niacinamide on H₂O₂-induced oxidative stress. (A) Protective effect of niacinamide on H₂O₂-induced ROS generation. Niacinamide (1, 2 and 4 mM) was treated with 1 mM H₂O₂ for 30 min. After treatment, cells were stained with DCF-DA probe for 30 min and fluorescent images were obtained. The scale bar represents 200 μm. (B) ROS generation was quantitated using Celleste™ Image Analysis Software. (C) LDH release was assessed in hDPCs following H₂O₂ exposure for 24 h. (D) Protective effect of niacinamide on H₂O₂-induced LDH release. Niacinamide (1, 2 and 4 mM) was treated with 750 µM H₂O₂ for 24 h. Data represent mean ± S.D. ^#p < 0.05 and ^##p < 0.01 compared to non-treated control. *p < 0.05, **p < 0.01 compared to H₂O₂-treated control.

Abbreviations: N.T, non-treated control; NIA, niacinamide; MNX; minoxidil.