FIG. 2.

PPARα and PGC-1 cooperate to induce PPARα gene target markers of the mitochondrial FAO pathway. Autoradiographs of Northern blot analysis performed with total RNA (15 μg) isolated from 3T3-L1 preadipocytes infected with recombinant retroviral particles encoding LacZ, PPARα, PGC-1, or PPARα and PGC-1 as indicated at the top are shown. Cells were grown to confluence and induced to differentiate as described in Materials and Methods. Six days after addition of differentiation media, ETYA (+) or vehicle control (−) was added. RNA was isolated 48 h after addition of ligand or vehicle. The blot was hybridized with the radiolabeled cDNA probes indicated on the right. The ethidium bromide (EtBr)-stained RNA is included as a control for loading and RNA integrity.