Figure 7.

Spreading depolarization inhibition by retigabine in in vivo anaesthetized wild-type mouse cortex. Spreading depolarization was triggered by repetitively applying a KCl solution (100, 300, 500, 1000 mM for 2 min) to the cortical surface. (A) Spreading depolarization wave was detected with IOS signal shown in a pseudocoloured image, and (B) electrophysiologically with Ag/AgCl electrode. (C) Summary of spreading depolarization threshold measurement. In each animal, spreading depolarization threshold was measured before and after drug injection. Vehicle (0.1% DMSO) and 10 mg/kg retigabine had no effect, while 30 mg/kg retigabine significantly increased K⁺ evoked spreading depolarization threshold. ****P < 0.001. (D) Number of recurrent spreading depolarization events during continuous 0.5 M KCl application for 30 min. Retigabine 30 mg/kg significantly decreased the regenerative spreading depolarization number. *P < 0.05, **P < 0.01.