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. 2021 Mar 26;144(9):2863–2878. doi: 10.1093/brain/awab141

Figure 8.

Figure 8

Kv7.2 activator retigabine inhibits mild, but not severe OGD-induced spreading depolarization. (A) Image of OGD-spreading depolarization wave in cortical slice triggered by continuous exposure to OGD solution (0% O2, 0 mM glucose), and detected as IOS travelling across cortical tissue. (B) Arrival of IOS signal (top) near electrode coincided with negative DC potential shift (bottom trace). (C and D) Retigabine delayed OGD-spreading depolarization when metabolic stress was mild (2 mM glucose), but had no effect during severe compromise (0 mM glucose). Retigabine did not inhibit spreading depolarization propagation rate (D) n = 8 each, *P < 0.05. (E–G) Retigabine had no effect on OGD-spreading depolarization generated in slices of the medulla at the level of the nucleus tractus solitarius (nTS). (E) Spreading depolarization was triggered by 0% O2/5 mM glucose solution with or without 30 µM retigabine. (F and G) Spreading depolarization onset was determined by the IOS signal peak at the lateral margin of the nTS. (n = 7 in all experiments). SD = spreading depolarization.