Figure 4.

Effects of muscle-specific expression of mutant Drosophila LamC transgenes on larval motility and adult viability were analyzed. (A) Diagram of lamin A/C showing domains and location of the amino acid substitutions under study. (B) Velocity measurements were made on the host stock and transgenic larvae expressing either wild-type or mutant LamC exclusively in the larval body-wall muscles. For the transgenic larvae, the amino acid substitution is indicated along the X-axis; parentheses represent the strain designation). Velocity values are expressed as the mean ± standard deviation. n = 13–15 larvae per genotype. LamC R564P was previously shown to reduce motility and was used as a positive control. **** p < 0.0001 (C) Distance per contraction of third instar larvae were measured for the host stock and transgenic larvae expressing either wild-type LamC or mutant LamC exclusively in the larval body-wall muscles. Distance per contraction measurements are expressed as the means ± standard deviation. n = 13–15 larvae per genotype. * p < 0.05, **** p < 0.0001 (D) The percent adult viability was measured for the host stock and transgenic flies with larval body-wall muscle-specific expression of mutant LamC. Ø indicates zero adults were viable. n = 174–426 adults per genotype. **** p < 0.0001.