Figure 4.

Disruption of Tat–TAR RNA interaction by T0516-4834 compound. (A) Structures of TAR RNA and bulge-deleted (delta) TAR RNA. (B,C) Immunoblotting of Tat bound to TAR RNA. Tat and TAR RNA binding was performed using protein lysate prepared from 293T cells expressing Flag-Tat and biotinylated TAR RNA coupled to agarose beads. Where indicated, the incubation mixture was supplemented with 10 µM of the indicated compound. Tat protein was eluted from the beads with SDS loading buffer, resolved on SDS-PAGE and detected with anti-Flag antibody. (C) Quantification of Tat protein bound to TAR RNA. Shown are results from three independent experiments that were plotted using Prism 6. * p = 0.04 and ** p = 0.001 in comparison to the DMSO controls.