Figure 6.
Levels of the anti-PEDV-specific immunoglobulin G (IgG) antibody (a) and the anti-PEDV-neutralizing antibody titer (b) in mice post-immunization. The mice were divided into four groups with 30 in every group. The results were ascertained by measuring the anti-PEDV IgG antibody and the anti-PEDV-neutralizing antibody titer with 2 × serial dilution in sera (dilution in 5% skimmed milk) and intestinal mucus (dilution in PBS) from immunized mice by indirect ELISA (a) using the PEDV as the coating antigen and virus neutralization experiment (b). ** p < 0.01 compared to the control groups.