Figure 4.

Expression and localization of Apt1p in S. cerevisiae. ZHY709 cells were transformed with an empty plasmid vector (E.V.) or with plasmids encoding the indicated Apt1p/Cdc50p variants. Gene expression was induced by growth in galactose medium at 25 °C. (A) Representative fluorescence (GFP) or bright-field (BF) confocal images. DAPI (blue) was used to identify nuclei. Scale bars, 5 µm. (B) In-gel fluorescence (lower panel) and Western blot analysis (upper panel) of total protein extracts or total membrane preparations. Blots were stained with anti-FLAG antibody. The mobilities of marker proteins of known mass (kDa) are indicated on the left.