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Live/dead staining assay using calcein AM and ethidium homodimer-1 dyes. CCD-18Co cells were incubated in presence of PEO-IONPs and GM3-IONPs for 24 and 48 h (Concentration—500 μg/mL). Live cells appear green in color and dead cells appear red in color. All the images were merged together for both green and red channel filters of the microscope. (A,B)—control cells (no IONPs); (C,D)—cells exposed to PEO-IONPs; (E,F)—cells exposed to GM3-IONPs. Magnification: 100×; Scale bar—100 μm.
